Applications of Lentiviral Vectors for shRNA Delivery and Transgenesis
نویسندگان
چکیده
منابع مشابه
Lentiviral vectors: are they the future of animal transgenesis?
Lentiviral vectors have become a promising new tool for the establishment of transgenic animals and the manipulation of the mammalian genome. While conventional microinjection-based methods for transgenesis have been successful in generating small and large transgenic animals, their relatively low transgenic efficiency has opened the door for alternative approaches, including lentiviral vectors...
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Microinjection of DNA is now the most widespread method for generating transgenic animals, but transgenesis rates achieved this way in higher mammals are extremely low. To address this longstanding problem, we used lentiviral vectors carrying a ubiquitously active promoter (phosphoglycerate kinase, LV-PGK) to deliver transgenes to porcine embryos. Of the 46 piglets born, 32 (70%) carried the tr...
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We have previously shown that lentiviral vectors can be used to generate transgenic chickens efficiently and that expression of introduced transgenes is not silenced on transmission through the germline. Transgene expression can be targeted in the predicted tissue-restricted manner, for example: muscle-specific expression is restricted to skeletal muscle using the rat myosin light chain 3 gene ...
متن کاملApplications of lentiviral vectors in molecular imaging.
Molecular imaging provides the ability of simultaneous visual and quantitative estimation of long term gene expression directly from living organisms. To reveal the kinetics of gene expression by imaging method, often sustained expression of the transgene is required. Lentiviral vectors have been extensively used over last fifteen years for delivery of a transgene in a wide variety of cell type...
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Recent years, allogeneic bone marrow transplantation (BMT) has proved to be the successful cure for patients with thalassemia major, however this is restricted due to limited matched-related donor. Its complications include chronic graft-versus-host disease in 5-8% of patients. So, a molecular approach, such as gene therapy for direct normal beta globin gene transmission, seems quite promising ...
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ژورنال
عنوان ژورنال: Current Gene Therapy
سال: 2008
ISSN: 1566-5232
DOI: 10.2174/156652308786848067